实验性自身免疫性脑脊髓炎模型(EAE模型)
背景简介:多发性硬化症(Multiple Sclerosis,MS)是一种发生于中枢神经系统的炎症性脱髓鞘疾病。实验性自身免疫性脑脊髓炎模型(Experimental Autoimmune Encephalomyelitis,EAE)是研究该疾病的基本模型。EAE模型实验动物通过神经组织(其中的某些成分)或病毒诱导产生的。EAE的诱导有主动诱导法和被动诱导法两种。不同的免疫方法,可影响抗原递呈,激发不同的免疫反应,这不但影响EAE的发生率,还可影响其发展与转归。通常采用主动诱导法制备EAE模型,将抗原与佐剂的混合乳剂直接注射至动物体内,经过一定时间的潜伏期,诱导EAE的产生。抗原注射部位的不同,可影响EAE的发生率、潜伏期、死亡率及复发率。常用的注射部位有脚垫、颈部及背部(皮下、皮内、肌肉)、腹腔和尾静脉等多种,其中以皮下多点注射及脚垫注射的发病率最高,而颈部注射则有较高的复发率。 因多次注射易引发免疫耐受,现在多采用单次注射抗原法。EAE的被动转移实验是研究其发病机制的最直接证据,也是研究EAE的治疗及预防措施的良好模型。
操作流程:小鼠品系:雌性C57BL/6 小鼠
周龄:6-8周
0天:等体积混合MOG35-55(200ug)和完全佐剂(CFA,0.5mg)配成乳剂 ,皮下注射100ul/只。
3天:腹腔注射200ng 无菌PBS溶解的百日咳毒素,100ul/只。
典型的EAE发病时间为免疫后9-14天,免疫后3-5天为高峰期,随后逐渐恢复。高峰期持续1-3天。有25%概率的小鼠在免疫恢复后,疾病复发,严重程度增加,通常发生在免疫后的20-27天。小鼠通常观察4周左右,在此期间,小鼠将保持慢性瘫痪。
此方案源于文献(PMID:
20811645),具体的免疫剂量我们建议客户根据您的实验需求制定和优化实验方案。以上方案仅供参考。
EAE的神经症状通常根据Kono等提出的标准分为5级。0级:无任何临床症状;1级:动物尾巴无力,2级:尾部无力+肢体无力,3级:肢体轻度麻痹,4级:肢体严重麻痹,被动翻身后不能复原;5级:濒死状态或死亡。
应用文献:1.Shibamura-Fujiogi, etal.Cathepsin L regulates pathogenic CD4 T cells in experimental autoimmune encephalomyelitis.April 1, 2021,International Immunopharmacology.
2.Shen, PX,etal.Urolithin A ameliorates experimental autoimmune encephalomyelitis by targeting aryl hydrocarbon receptor.January 30, 2021,Ebiomedicine
3.Takeshita,etal.IL-6 blockade suppresses the blood-brain barrier disorder, leading to prevention of onset of NMOSD.January 28, 2021,Biorxiv.
4.Wei, L,etal.Arctigenin Exerts Neuroprotective Effect By Ameliorating Cortical Activities in Experimental Autoimmune Encephalomyelitis in Vivo.January 25, 2021,Research Square.
5.Kim, TW,etal.Bilirubin nanomedicine ameliorates the progression of experimental autoimmune encephalomyelitis by modulating dendritic cells.January 12, 2021,Journal Of The Controlled Release.
6.Yang, R,etal.Diffusion Basis Spectrum Imaging Measures Anti-Inflammatory and Neuroprotective Effects of Fingolimod on Murine Optic Neuritis.January 9, 2021,Research Square.
7.Mimouna, S,etal.Transcription cofactor GRIP1 differentially affects myeloid cell-driven neuroinflammation and response to IFN-β therapy.January 4, 2021,The Journal Of Experimental Medicine.
8.Li, PY.PEGylation enables subcutaneously administered nanoparticles to induce antigen-specific immune tolerance.January 12, 2021,Journal Of Controlled Release.
9.Aqel, SI,etal.A STAT3 inhibitor ameliorates CNS autoimmunity by restoring Teff:Treg Balance.January 7, 2021,JCI Insight.
10.Chaudhary, P,etal.Thyroid hormone and thyromimetics inhibit myelin and axonal degeneration and oligodendrocyte loss in EAE.December 27, 2020,Journal Of Neuroimmunology
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货号 |
英文名称 |
规格 |
Hooke Labs |
DS-0111 |
Rat/Mouse Mog35-55 |
2mg/ml,1ml |
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Pertussis Toxin, Lyophilized in buffer |
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