Mouse Anti col2A1 Antibody (M2139)

Mouse Anti col2A1 Antibody (M2139)
cat#10110
 

Affinity purified mouse monoclonal anti collagen II (COL2A1) antibody clone M2139 in PBS, sterile filtered (20 µm). The antibody recognizes the J1 epitope on triple helical collagen II¹. The J1 epitope on mouse collagen consist of residues 551–564 with sequence MPGERRGAAGIAGPK^1,4. The antibody M2139 has been used in applications including immunohistochemistry⁴, Western blot², immunoprecipitation³, and in vivo⁴ and in vitro⁵ functional assays.

Target with alternative names: Collagen alpha-1(II) chain, COL2A1, type II collagen, CII; UniProt: P28481.
Immunogen: Mouse collagen II J1 epitope (triple helical position 551-564) with sequence MPGERRGAAGIAGPK^1,4.
Species reactivity: Mouse, rat, human, bovine, chicken
Isotype: Mouse IgG2b, κ
Specificity: The antibody M2139 recognizes the conformational J1 epitope on the triple-helical structure of the native CII molecule.
Concentration: 1 mg/ml
Supplied in: PBS
Storage: Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at 20°C long term. Avoid repeated freeze/thaw cycles.
Recommended dilution: It is recommended the user determines the optimal dilution for their application. The typical starting working dilutions are: Functional assays (cell culture) 1:20–1:50, IHC 1:100.