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细胞分离检测试剂
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光诱导蛋白激活剂 Actiflash

光诱导蛋白激活剂 Actiflash

Actiflash

报价:23205.00

联系购买
中文名称:
光诱导蛋白激活剂 Actiflash
英文名称:
Actiflash
品牌:
Idylle
货号:
LJU-CIN-50
规格:
50mg
别名:
Actiflash

How does it work?

Actiflash is a tamoxifen-like caged-inducer called cyclofen. Upon UV-light illumination, optical-induced uncaging of Actiflash quickly releases proteins fused to the modified estrogen receptor ligand-binding domains ERT2. This allows them to translocate into the nucleus, activate transcription (using Gal4-UAS system) or induce recombination (using the Cre-lox one).


The protein expression can thus be simply controlled in time and space thanks to UV-light!




Applications
Actiflash is a great R&D tool to convert your inducible ERT model into a photo-inducible one. It is very helpful if you want to control transcription (using Gal4-UAS) or induce recombination (using Cre-lox) in space and/or time for in-vivo cell tracking experiments and more.

Actiflash has been successfully used with various cell lines as well as zebrafish embryos. Actiflash will work best on zebrafish embryos from 12 to 48hpf. 

Find below a plasmid list that can be used with Actiflash. All these plasmids are available from Addgene.

 

Technical Information
Lifetime: upon receipt, Actiflash is stable for years at 2-8°C. Once solubilized in a DMSO solution (typically at 10 mM), Actiflash can be stored at -20°C for several months.  Always wrap the vials containing the aliquoted Actiflash with aluminium foil to protect it from light
 

 Additional resources:

>  ​Product overview
>  Safety Datasheet
>  Certificate of Analysis
 

Visit our  glossary  for a definition of Tamoxifen.


产品订购:
产品名称 货号  规格
光诱导蛋白激活剂 Actiflash LJU-CIN-5 5mg
光诱导蛋白激活剂 Actiflash LJU-CIN-50 50mg

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