ColorFlux is a fast, reliable and non-toxic solution for assessing bacterial efflux for your research on antibiotic resistance. This coloured fluorescent compound quickly accumulates within bacteria as a function of their efflux pump activity. Simply add ColorFlux to your bacteria and watch them change colour !
How does ColorFlux work?
ColorFlux staining was shown to reflect the activity of well-characterized efflux pumps from the major facilitator superfamily and ATP-binding cassette families in a variety of Gram+ and Gram- bacteria:
- NorA, MepA, MepB, PatA, PatB (Staphylococcus aureus & Streptococcus pneumoniae)
- BmrA (Bacillus subtilis)
- AcrAB (Haemophilus influenzae)
The list will be updated regularly according to the feedback of users.
Technical information about ColorFlux
Tested and validated on : Staphylococcus aureus, Bacillus subtilis, Streptococcus pneumoniae, Haemophilus influenzae.
Colour: Red
Form: liquid
Detection methods: visual & fluorescence. Also compatible with single-cell approaches (live imaging and flow cytometry)
Peak excitation/emission wavelengths: Ex:530nm/Em:650nm
Storage: at 4°C protected from light for 8 months
1 kit contains 1mL of an aqueous solution with a concentration of 1 mg/mL for a final volume of 1L of screening medium
Results:
ColorFlux staining of MepA&B mutants in Staphylococcus aureus
ColorFlux staining of NorA mutants in Staphylococcus aureus
ColorFlux staining of PatA/PatB mutants in Streptococcus pneumoniae
ColorFlux staining of BmrA mutants in Bacillus Subtilis
Distinguishing efflux activity levels across H. influenzae strains
Bacillus subtilis bacteria (moderate activity) stained with ColorFlux
产品订购：
| 中文名称 |
产品名称 |
货号 |
规格 |
| 细菌外排指示剂 |
ColorFlux（细菌外排指示剂） |
OSI-STA-1000 |
1mL |
| 细菌外排指示剂 |
ColorFlux（细菌外排指示剂） |
OSI-STA-250 |
250 µL |
