Immunization Grade Bovine Type XI Collagen, 5 mg, lyophilized

DESCRIPTION: Type XI collagen purifi ed from pepsin-solubilized articular cartilage by repeating salt precipitation. Type XI collagen is one of three types of collagen composing cartilage fi brils and consists of three α-chains, α1 (XI), α2 (XI), and α3 (XI), where α3 (XI) is homologous to α1 (II) chain of type II collagen (1). Note: Type XI collagen shares substantial similarity with type V collagen, which consists of α1 (V), α2 (V), and α3 (V) chains, but these alpha chains are not identical (2).
APPLICATION: Use as an immunizing antigen to generate antibodies, an antigen to detect anti-type XI collagen antibodies in ELISA, or as a standard for gel analysis.
Note: Antibodies against to type II collagen partially cross-react to type XI collagen due to the homology of α3 (XI) to α1 (II).
QUANTITY: 5 mg
FORM: Lyophilized powder
SOURCE: Bovine
MOLECULAR WEIGHT: Intact type XI collagen: approximately 360 Kd. By 6% gel analysis, type XI collagen is separated into three α-chains, α1 (XI), α2 (XI), and α3 (XI) (1052, 1478, and 1060 A.A. residues) from the top of the gel.
PURITY: >90% by SDS-PAGE gel analysis
SOLUBILITY: Type XI collagen can be dissolved at 4 mg/ml in acidic solution such as 0.01-0.05M acetic acid, pH 3.0-3.3 or 0.15M citrate buffer, pH 3.6 by stirring at 4°C overnight. To neutralize the solution, add 10X neutral buffer containing 1.5M NaCl or dialyze the solution against a neutral buffer.
STORAGE TEMPERATURE: 4°C in the dark for lyophilized form and –20°C for solution. Collagen might be gradually degraded under neutral conditions.
STABILITY: 2 years in lyophilized form
REFERENCES: 1. Von der Mark K, Van Menxel M, Wiedemann H. Isolation and characterization of new collagens from chick cartilage. Eur. J. Biochem. 124: 57-62 (1982)
2. Burgeson RE, Hebda PA, Morris NP and Hollister DW. Human cartilage collagens. Comparison of artilage collagens with human type V collagen.