Immunization Grade Porcine Type XI Collagen, 5 mg, lyophilized

DESCRIPTION: Type XI collagen is purified from pepsin-solubilized articular cartilage by repeated salt precipitation. Type XI collagen is one of three types of collagen which make up cartilage fibrils and consists of three a-chains, a1 (XI), a2 (XI), and a3 (XI), where a3 (XI) is homologous to the a1 (II) chain of type II collagen (1). Note: Type XI collagen shares significant similarities with type V collagen, which consists of a1 (V), a2 (V), and a3 (V) chains, but these alpha chains are not identical (2).
APPLICATION: Use as an immunizing antigen to generate antibodies, an antigen to detect anti-type XI collagen antibodies in ELISA, or as a standard for gel analysis.
Note: Antibodies against type II collagen partially cross-react to type XI collagen due to the homology between a3 (XI) and a1 (II).
QUANTITY: 5 mg
FORM: Lyophilized powder
SOURCE: Porcine
MOLECULAR WEIGHT: Intact type XI collagen, approximately 360 kDa. By 6% gel analysis, type XI collagen is separated into three a-chains: a1 (XI), a2 (XI), and a3 (XI) (1052, 1478, and 1060 A.A. residues)
PURITY: >90% by SDS-PAGE gel analysis
SOLUBILITY: Type XI collagen can be dissolved at 4 mg/ml in acidic solution, such as 0.01-0.05M acetic acid (pH 3.0-3.3) or 0.15M citrate buffer (pH 3.6), by stirring at 4°C overnight. To neutralize the solution, add 1/10 volume of a 10X concentrated neutral buffer containing 1.5M NaCl or dialyze the solution against a neutral buffer containing 0.15M NaCl.
STORAGE TEMPERATURE: 4°C in the dark. After reconstitution, store at–20°C. Collagen may degrade under neutral conditions.
STABILITY: 2 years
REFERENCES: 1. Von der Mark K, Van Menxel M, Wiedemann H. Isolation and characterization of new collagens from chick cartilage. Eur. J. Biochem. 124: 57-62 (1982)
2. Burgeson RE, Hebda PA, Morris NP and Hollister DW. Human cartilage collagens. Comparison of artilage collagens with human type V collagen.