Anti-Mouse Type I Collagen Antibody, Clone 7G5D2, Biotinylated

DESCRIPTION: Rat monoclonal anti-mouse type I collagen antibody clone 7G5D2 (IgG2a), biotinylated

APPLICATION: Use for immunohistochemistry and ELISA, but not recommended for immunoblotting

QUANTITY: 100 ml, 1 mg/ml

FORM: Dissolved in PBS, pH 7.2

SOURCE: Rat

CROSS-REACTIVITY: Specific to native type I collagen and poorly reacts with denatured type I collagen (less than 5%). Does not cross-react with any species of type II collagen. The following is a list of relative cross-reactivity with various species of type I collagen.

                                 Rat type I collagen: 30%
                                 Mouse type I collagen: 20%
                                 Bovine type I collagen: 50%
                                 Porcine type I collagen: 100%
                                 Human type I collagen 0%

USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as NGS, to prevent non-specific reactions in ELISA and tissue staining. Use at 0.1-1 mg/ml.

STORAGE TEMPERATURE: -20°C

STABILITY: 1 year