Anti-Type II Collagen (CB11) Antibody, Clone D1-2G, Biotinylated

DESCRIPTION: Mouse monoclonal anti-type II collagen (CB11) IgG2b antibody, clone D1-2G, biotinylated

APPLICATION: Use for immunohistochemistry, immunoblotting, and ELISA

QUANTITY: 100 μl, 1 mg/ml

FORM: Solution in PBS

SOURCE: Mouse

PURITY: Purified by affinity chromatography

CROSS-REACTIVITY: Cross-reacts to most species of type II collagen including mouse, porcine, chick, bovine, human, rat, monkey, rabbit, equine, and dog. Cross-reactivity with denatured type II collagen is minimal (less than 10 %)

EPITOPE: LyC1 of CB11 on type II collagen

SUBTYPE: IgG2b

USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as NGS, to prevent non-specific reactions in ELISA, tissue staining or immunoblotting. Use 0.1-1 mg/ml. Due to low reactivity to denatured collagen, ten times higher concentration will be required to detect denatured collagen in immunoblotting.

STORAGE TEMPERATURE: -20°C

STABILITY: 1 Year

REFERENCES: 1. K. Terato et al., Induction of arthritis with monoclonal antibodies to collagen. J Immunol 148, 2103- 2108 (1992).
                       2. P. Hutamekalin et al., Collagen antibody-induced arthritis in mice: development of a new arthritogenic 5-clone cocktail of monoclonal anti-type II collagen antibodies. J Immunol Methods 343, 49-55 (2009).