Anti-Type II Collagen (CB11) Antibody, Clone K4-5C

DESCRIPTION: Mouse anti-chick type II collagen (CB11) IgG3 monoclonal antibody, clone K4-5C, unconjugated

APPLICATION: Use for immunohistochemistry, immunoblotting, and ELISA

QUANTITY: 100 μl, 1 mg/ml

FORM: Mouse monoclonal antibody, clone K4-5C, recognizing the conserved epitopes on various species of type II collagen. Dissolved in PBS, pH 7.2.

SOURCE: Mouse

CROSS-REACTIVITY: Cross-reacts to most species of type II collagen including porcine, chick, bovine, human, rat, and monkey.

USAGE: Dilute the antibody in buffer containing 2% heterologous serum, such as NGS, to prevent non-specific reactions in ELISA, tissue staining or immunoblotting. Use 0.1-1 mg/ml. Due to low reactivity to denatured collagen, ten times higher concentration will be required to detect denatured collagen in immunoblotting.

STORAGE TEMPERATURE: -20°C

STABILITY: 1 year

REFERENCES: Terato K. et al. J. Immunol. 148: 2103-2108 (1992)