T-Cell Grade Rat Type II Collagen, 0.5 mg, lyophilized

DESCRIPTION: Highly purified by CM-cellulose chromatography, heat denatured type II collagen
APPLICATION: Use as a stimulation antigen for T-cells from experimental animals and humans in vitro culture system, for example, T-cell proliferation assay and establishing type II collagen specific T-cell lines.
QUANTITY: 0.5 mg
FORM: Lyophilized powder
SOURCE: Rat articular cartilage
MOLECULAR WEIGHT: 300 Kd
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans
SOLUBILITY: Soluble in acidic and neutral buffers, and in cell culture medium. In order to use this product for assaying T-cell proliferation responses, it is recommended to dissolve this product in PBS rather than cell culture media at 1 mg/mL before use.
STORAGE TEMPERATURE: 4°C in the dark. This product is stable at 4°C for 2 years as supplied. Denatured collagen dissolved in acidic solution is also stable at -20°C for 1 year, but will gradually degrade under neutral conditions.
STABILITY: 2 years
REFERENCES: Trelstad R. et al. Biochemistry 9: 4993-4998 (1970)
Miller EJ. Biochemistry 10: 1652-1659 (1971)
Terato K. et al. Brit. J. Rheum. 35: 828-838 (1996)